home *** CD-ROM | disk | FTP | other *** search
- -- card: 8258 from stack: in.1
- -- bmap block id: 0
- -- flags: 4000
- -- background id: 3161
- -- name:
- ----- HyperTalk script -----
- on openCard
- set the lockText of field "BufferInfo" to true
- set the visible of field "helptext" to false
- put line 1 of field BufferInfo into field Name
- end openCard
-
-
- -- part contents for background part 30
- ----- text -----
- Tris
- pKa is 8.06
- dpK/dT is -0.028
- charge on acid species is 1
- made from Tris_base
- Mol.wt. is 121.14
- Shortname is Tris
- Notes: very temperature sensitive!
-
- -- part contents for background part 11
- ----- text -----
- Tris
-
- -- part contents for background part 15
- ----- text -----
- 37.4
-
- -- part contents for background part 59
- ----- text -----
- 20
-
- -- part contents for background part 43
- ----- text -----
- 1000
-
- -- part contents for background part 12
- ----- text -----
- 8
-
- -- part contents for background part 14
- ----- text -----
- .3
-
- -- part contents for background part 10
- ----- text -----
- Buffer recipe Monday, November 13, 1989
-
- Buffer : .3M Tris pH 8 at T=37.4 °C
- pH: 8.487 at lab temperature of 20 °C
-
- T: 37.4°C
- pKa: 8.06, pKa': 7.836 (dpKa/dT: -0.028)
- I: 0.122 (Ionic strength due to buffer components)
- Notes: very temperature sensitive!
-
- 1. Dissolve 36.342g Tris_base
- in ~800.000 ml double distilled water.
-
- 2. Titrate to pH 8.487 with monobasic acid or alkali.
-
- 3. Make up to 1000mls
-
-